High Quality Content by WIKIPEDIA articles! Two-dimensional gel electrophoresis (2-DE) is used to separate complex protein mixtures by first separating the proteins according to their isoelectric point (pI) and then according to their molecular weight. This separation would then enable the scientist to identify the proteins according to their published pI and MW values or subject them to further separation or identification using Mass Spectrometry. 2-DE is a time consuming procedure resulting in copious amounts of raw data. Thus in order use computational means to analyze the data in context, meaning full data structure are required/needed. These data standards are slow in coming due to complexity and integrative nature of knowledge required to develop this is sparse. Annotated Gel Markup Language, or AGML is an attempt to bridge this gap.