In biochemistry, chemosynthesis is the biological conversion of one or more carbon molecules (usually carbon dioxide or methane) and nutrients into organic matter using the oxidation of inorganic molecules (e.g. hydrogen gas, hydrogen sulfide) or methane as a source of energy, rather than sunlight, as in photosynthesis. Chemoautotrophs, organisms that obtain carbon through chemosynthesis, are phylogenetically diverse, but groups that include conspicuous or biogeochemically-important taxa include the sulfur-oxidizing gamma and epsilon proteobacteria, the Aquificaeles, the Methanogenic archaea and the neutrophilic iron-oxidizing bacteria. Many microorganisms in dark regions of the oceans use chemosynthesis to produce biomass from single carbon molecules. Two categories can be distinguished.

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