Electrochemical synchronization of cell spreading on solid surfaces. Dictyostelium discoideum: synchronization of spreading

Electrochemical synchronization of cell spreading on solid surfaces. Dictyostelium discoideum: synchronization of spreading

Marius Socol

     

бумажная книга



Издательство: Книга по требованию
Дата выхода: июль 2011
ISBN: 978-3-8433-6063-0
Объём: 168 страниц
Масса: 276 г
Размеры(В x Ш x Т), см: 23 x 16 x 1

Cell synchronization is important for the analysis of molecular events involved in cell spreading and motility. Electrostatic interactions between cells and surfaces were investigated in order to synchronize the first step in cell adhesion. LimE- GFP marked Dictyostelium discoideum cells were used for fluorescent tracking of actin polymerization events. Oscillating LimE fluorescent peaks were observed for individual cells in standard phosphate buffer during spreading. At low ionic concentration (phosphate sucrose buffer 0.17 mM), cells levitate over the conductive surfaces (Indium Tin Oxide, ITO) due to electrostatic repulsion. An electrochemical device was designed in order to apply an overpotential pulse (+2.5 V/Ag,AgCl) during 0.1 s to the ITO surface. In these conditions, protons are produced by water oxidation, which reduce the ITO negative surface charge and thus, attracting the levitating cells simultaneously. Consequently, these irreversible contacts with the surface triggered the onset of cell spreading. Therefore, we obtained synchronization of the spreading of a cell population for the first time thanks to an electrochemical method.

Данное издание не является оригинальным. Книга печатается по технологии принт-он-деманд после получения заказа.