High Quality Content by WIKIPEDIA articles! HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants of many other buffers do not change much with temperature. HEPES is like water in that its dissociation decreases as the temperature decreases.

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