Indoor environment is vital for human health. Two university problem buildings were compared with two control buildings. Health assessment included tear film, nasal patency, nasal lavage analyses and markers for allergy and atopy. Psychosocial and physical environment was assessed with questionnaires, inspections, air microbiology, thermal climate and air quality at 56 points and modelled for all work sites. Impact of gender differences, allergy and home environment were also studied. Exposure differences between problem buildings and controls were small, variations between rooms were greater. Workers in the problem buildings had more general and dermal symptoms, but not more objective signs. Tear film stability through the work day was impaired by low night air temperatures and low relative air humidity. Low air humidity and air velocity were associated with perceiving dry air and feeling too cold. Low night indoor air temperature, increased air temperature difference between day and night and fast changes in air temperature might impair indoor environment. This may have implication for choice of energy saving policies in buildings.

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