ISBN: | 978-5-5136-5992-1 |
High Quality Content by WIKIPEDIA articles! Interference reflection microscopy or IRM is an optical microscopy technique that utilizes polarized light to form an image of an object on a glass surface. The intensity of the signal is a measure of proximity of the object to the glass surface. This technique can be used to study events at the cell membrane without the use of a (fluorescent) label in contrast to TIRF microscopy.