Single strand conformational polymorphism (SSCP) is a technique which is used to detect point mutation in genes. The purpose was to optimize the condition of SSCP for efficient and sensitive mutation detection in Exon 4, 5, 6, 7, 8, 9, 10, 11 and 12. All these Exon were amplified and run on agarose gel to check strong and specific amplification. These samples were then subjected for vertical electrophoresis for SSCP using different conditions of gel concentration, time, temperature and voltage to get maximum separation between single strands. Denaturation was carried out by mixing the samples with denaturant reagents. The length of nucleotide of Exon 4 was much longer than normal, so we amplified the exon 4 into two small parts named as 4a and 4b respectively after its bifurcation. The denaturation reagent consist of 50% 100mM NaOH and 50% Formamide loading buffer, 5 l-10 l sample was loaded in each case. In all the optimized Exons the denaturation was performed at 95°C for 7 minutes in PCR machine. This optimization will prove a valuable help in further study of the Exons of the same gene and other genes for Point Mutation Detection.
Данное издание не является оригинальным. Книга печатается по технологии принт-он-деманд после получения заказа.
Mobile Technologies have become ubiquitous and pervasive coupled with its networked and enhanced capabilities for rich social interactions, they are well suited in engaging people in personal learning and for collaborative experiences. The rise in mobile technologies such as internet capable smart phones with rich media capabilities presents an opportunity for educational contents that can aid informal learning. Majority of students spent part of their time using these mobile technologies such as laptops and smart phones, hence there is the need to consider how the possibilities of these internet capable smart phones can help students to learn better. This project work focuses on the design, implementation and evaluation of a mobile collaborative learning system prototype that will enable people especially students to learn and collaborate.
Данное издание не является оригинальным. Книга печатается по технологии принт-он-деманд после получения заказа.
