High Quality Content by WIKIPEDIA articles! Prefoldin is a family of proteins used in protein folding complexes. It is classified as a heterohexameric molecular chaperone in both archaea and eukarya, including humans. A prefoldin molecule works as a transfer protein in conjunction with a molecule of chaperonin to form a chaperone complex and correctly fold other nascent proteins. One of prefoldin`s main uses in eukarya is the formation of molecules of actin for use in the eukaryotic cytoskeleton.