High Quality Content by WIKIPEDIA articles! Radiation reduced hybrid is procedure for discovering the location of genetic markers relative to one another. The relative location of these markers can be combined into a physical map or a genetic map. The radiation hybrid technique begins as another way to amplify and purify DNA, the first step in any sequencing project; radiation is used to break the DNA into pieces and these pieces are incorporated into the hybrid cell; the hybrid can be grown in large quantities. One can then check for the presence of various genetic markers using PCR and linkage analysis to resolve the distance between the markers. That is, if two genetic markers are near each other, they are less likely to be separated by the DNA-breaking radiation. The technique is similar to traditional linkage analysis, where we depend on genetic recombination to calculate the distance between two genetic markers.

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