Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends

Jesse Russell Ronald Cohn

     

бумажная книга



ISBN: 978-5-5122-5441-7

High Quality Content by WIKIPEDIA articles! Rapid Amplification of cDNA Ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR). The amplified cDNA copies are then sequenced and, if long enough, should map to a unique mRNA already described, the full sequence of which is known. RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript to the 5` end (5` RACE-PCR) or 3` end (3` RACE-PCR) of the RNA. This technique is sometimes called one-sided PCR or anchored PCR.