High Quality Content by WIKIPEDIA articles! Rhodanese is a mitochondrial enzyme which detoxifies cyanide (CN-) by converting it to thiocyanate (SCN-). This reaction takes place in two steps. The diagram on the right shows the crystallographically-determined structure of rhodanese. In the first step, thiosulfate reacts with the thiol group on Cysteine-247 1, to form a disulfide 2. In the second step, the disulfide reacts with cyanide to produce thiocyanate, itself being converted back into the "normal" thiol 1.

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