High Quality Content by WIKIPEDIA articles! Sanger sequencing is a method of DNA sequencing, based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. Developed by Frederick Sanger and colleagues in 1977, it was the most widely-used sequencing method for approximately 25 years. More recently, Sanger sequencing has been supplanted by "Next-Gen" sequencing methods, especially for large-scale, automated genome analyses. However, the Sanger method remains in wide use in 2012, primarily for smaller-scale projects and for obtaining especially long contiguous DNA sequence reads (>500 nucleotides).