High Quality Content by WIKIPEDIA articles! Thrombin is a "trypsin-like" serine protease protein that in humans is encoded by the F2 gene.Prothrombin (coagulation factor II) is proteolytically cleaved to form thrombin in the coagulation cascade, which ultimately results in the stemming of blood loss. Thrombin in turn acts as a serine protease that converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions.