High Quality Content by WIKIPEDIA articles! A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nm, can be observed. In cell and molecular biology, a large number of molecular events in cellular surfaces such as cell adhesion, binding of cells by hormones, secretion of neurotransmitters, and membrane dynamics have been studied with conventional fluorescence microscopes. To solve this problem, the TIRFM was developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s.

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