High Quality Content by WIKIPEDIA articles! U.S. Route 301 is a spur of U.S. Route 1 running through the South Atlantic States. It currently runs 1,099 miles (1,769 km) from Glasgow, Delaware at U.S. Route 40 to Sarasota, Florida. It passes through the states of Delaware, Maryland, Virginia, North Carolina, South Carolina, Georgia, and Florida. It goes through the cities of Annapolis, Maryland; Richmond and Petersburg, Virginia; Rocky Mount, Wilson, and Fayetteville, North Carolina; Florence, South Carolina; Statesboro and Jesup, Georgia; and Ocala, Zephyrhills, Brandon, and Sarasota, Florida.