High Quality Content by WIKIPEDIA articles! U.S. Route 41 (US 41) in Florida is a north–south United States Highway. It runs 480 miles (770 km) from Miami in South Florida northwest to the Georgia border north of the Lake City area. Within the state, US 41 is paralleled by Interstate 75 all the way from Georgia (on the northern border) to Miami, and I-75 has largely supplanted US 41 as a major highway.