Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Viable but nonculturable (VBNC) bacteria refers to bacteria that are in a state of very low metabolic activity and do not divide, but are alive and have the ability and become culturable once resuscitated. Bacteria in a VBNC state cannot grow on standard growth media, though flow cytometry can measure the viability of the bacteria. Bacteria can enter the VBNC state as a response to stress, due to adverse nutrient, temperature, osmotic, oxygen, and light conditions. The cells that are in the VBNC state are morphologically smaller, and demonstrate reduced nutrient transport, rate of respiration, and synthesis of macromolecules. Sometimes, VBNC bacteria can remain in that state for over a year. It has been shown that numerous pathogens and non-pathogens can enter the VBNC state, and therefore it has significant implications in pathogenesis, bioremediation, and other branches of microbiology.

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