Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. A vibratome is an instrument that is similar to a microtome but uses a vibrating razor blade to cut through tissue. The vibration amplitude, the speed, and the angle of the blade can all be controlled. Fixed or fresh tissue pieces are embedded in low gelling temperature agarose.(Some have had success without using the agarose to embed.) The resulting agarose block containing the tissue piece is then glued to a metal block and sectioned while submerged in a water or buffer bath. Individual sections are then collected with a fine brush and transferred to slides or multiwell plates for staining.

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